"We have been left with no choice but to pursue all legal avenues available to challenge the U.S. Department of Justice for its discriminatory enforcement activities against European online gaming operators," Clive Hawkswood, chief executive of the Remote Gambling Association (RGA), said in a statement.
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As part of a U.S. crackdown on Internet gambling, two founders of payments processor NETeller Plc were arrested in January. In May, BETonSPORTS pleaded guilty to U.S. racketeering charges and agreed to cooperate in a case against the company's founder and other co-defendants.
"The DOJ (Department of Justice) has repeatedly stated that all forms of online gambling are illegal, yet it continues to enforce this view only in connection with non-US businesses," the Internet gambling group said.
"As a result, the RGA has asked the EU to investigate the discriminatory enforcement regime as an illegal barrier to trade for EU businesses," the group said.